Coding

Part:BBa_K2055004:Design

Designed by: Yili Natalia Padilla   Group: iGEM16_Tec-Monterrey   (2016-10-12)


DNA coding for cyanide hydratase.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 287
    Illegal BamHI site found at 149
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 519


Design Notes

The coding sequence is optimized for its expression in C. violaceum.



Source

cyanide hydratase [Aspergillus niger CBS 513.88] NCBI Reference Sequence: XP_001389844.1

References